Principles and uses
Antibiotic Medium Nº 10 is the standard agar base used for the microbiological assay of antibiotics like polymixin. The activity (potency) of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on microorganisms. Reduction in antimicrobial activity may reveal changes not demonstrated by chemical methods. The antibiotic media are identified numerically with names assigned by Grove and Randall in “Assay methods of antibiotics”. The use of standardized culture media and strict control of all test conditions are essential requirements in the microbiological assay of antibiotics in order to obtain satisfactory test results. This medium is recommended by USP and the European pharmacopoeia. Polymixin assay is carried out using the cylinder plate method.
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